Journal: Biosensors

Article Title: Rapid Microfluidic Biosensor for Point-of-Care Determination of Rheumatoid Arthritis via Anti-Cyclic Citrullinated Peptide Antibody Detection

doi: 10.3390/bios14110545

Figure Lengend Snippet: Variation in OD intensity with anti-CCP Ab concentration for 2nd Ab-HRP reagents with different dilution factors. Error bars represent the range, calculated as the difference between the maximum and average values across three independent measurements.

Article Snippet: The anti-cyclic citrullinated peptide antibody (100 ng/mL = 30 IU/mL), sample diluent, and antibody diluent, as well as the 2nd antibody conjugate HRP (2nd Ab-HRP), were sourced from FineTest Inc. Additionally, 1% bovine serum albumin (BSA), diluted from a 10% stock solution was used as a blocking agent to reduce nonspecific binding, with phosphate-buffered saline (137 mM sodium chloride, 2.7 mM potassium chloride, 8.1 mM sodium hydrogen phosphate, 1.5 mM potassium dihydrogen phosphate), a wash buffer, and PBS containing 0.5% Tween 20 (PBST) used for rinsing steps.

Techniques: Concentration Assay